Product Name:
Rat Glucokinase (GCK) ELISA Kit
Cat.#:
JL21222
Regulatory Status:
Alternative:
GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase 4; Maturity Onset Diabetes Of The Young 2
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.34ng/mL
Specificity:
This kit recognizes Rat GCK in samples. No significant cross-reactivity or interference between Rat GCK and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| GCK
Glucokinase(GK) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. GK is a monomeric protein of 465 amino acids and a molecular weight of about 5 kD. Glucokinase occurs in cells in the liver and pancreas of humans and most other vertebrates. In each of these organs it plays an important role in the regulation of carbohydrate metabolism by acting as a glucose sensor. Mutations of the gene for this enzyme can cause unusual forms of diabetes or hypoglycemia.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 50 | 25 | 12.5 | 6.25 | 3.12 | 1.56 | 0.78 | 0 |
OD | 2.02 | 1.27 | 0.8 | 0.49 | 0.34 | 0.25 | 0.19 | 0.07 |
Corrected OD | 1.95 | 1.2 | 0.73 | 0.42 | 0.27 | 0.18 | 0.12 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Rat GCK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-101 | 96 |
Plasma(n=8) | 92-105 | 102 |
Tissue Homogenates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Rat GCK and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | Tissue Homogenates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
Average (%) | 91 | 93 | 96 | |
1 : 4 | Range (%) | 89-103 | 87-108 | 105-115 |
Average (%) | 94 | 99 | 108 |






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48T


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